Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.
We present a fast, reliable bostik mvp and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be google pixel 7 freedom carried out in one day.We show that EMP cloning has a higher efficiency than res